Photothermal nanoblade for patterned cell membrane cutting
نویسندگان
چکیده
We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized and specifically shaped explosive vapor bubble. Rapid bubble expansion and collapse punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. The membrane cutting pattern is controlled by the metallic nanostructure configuration, laser pulse polarization, and energy. Highly controllable, sub-micron sized circular hole pairs to half moon-like, or cat-door shaped, membrane cuts were realized in glutaraldehyde treated HeLa cells.
منابع مشابه
Dissection of the Burkholderia intracellular life cycle using a photothermal nanoblade.
Burkholderia pseudomallei and Burkholderia thailandensis are related pathogens that invade a variety of cell types, replicate in the cytoplasm, and spread to nearby cells. We have investigated temporal and spatial requirements for virulence determinants in the intracellular life cycle, using genetic dissection and photothermal nanoblade delivery, which allows efficient placement of bacterium-si...
متن کاملNanoblade delivery and incorporation of quantum dot conjugates into tubulin networks in live cells.
Quantum dots (QDs) have not been used to label cytoskeleton structure of live cells owing to limitations in delivery strategies, and QDs conjugation methods and issues with nonspecific binding. We conjugated tubulin to QDs and applied the emerging method of photothermal nanoblade to deliver QD-tubulin conjugates into live Hela cells. This method will open new opportunities for cytosolic target...
متن کاملMolecular Delivery into Live Cells with Light Image Patterns and a Gold Particle Coated Substrate
We present a method for permeabilizing cells using a disposable substrate coated with gold nanoparticles. Upon laser irradiation, the unique photothermal properties of the gold nanoparticles facilitate selective permeabilization of the cellular membrane using patterned laser images. The permeabilization of the cell membrane is characterized by intercalation of fluorescent Calcein dye.
متن کاملDigital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation.
Light-mediated neuromodulation techniques provide great advantages to investigate neuroscience due to its high spatial and temporal resolution. To generate a spatial pattern of neural activity, it is necessary to develop a system for patterned-light illumination to a specific area. Digital micromirror device (DMD) based patterned illumination system have been used for neuromodulation due to its...
متن کاملPhotothermal fabrication of microscale patterned DNA hydrogels
This paper introduces a method for fabricating microscale DNA hydrogels using irradiation with patterned light. Optical fabrication allows for the flexible and tunable formation of DNA hydrogels without changing the environmental conditions. Our scheme is based on local heat generation via the photothermal effect, which is induced by light irradiation on a quenching species. We demonstrate expe...
متن کامل